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odyssey infrared fluorescent scanner  (LI-COR)


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    Structured Review

    LI-COR odyssey infrared fluorescent scanner
    Odyssey Infrared Fluorescent Scanner, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 7084 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/odyssey+infrared+fluorescent+scanner/Odyssey+FC+Imaging+System/pmc13130404-157-29-33
    Average 99 stars, based on 7084 article reviews
    odyssey infrared fluorescent scanner - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Chemical proteomics reveals sinomenine’s anti-inflammatory mechanism through serum protein covalent modification
    Article Snippet: .. After mixture and denaturation by boiling for 5 min, the plasma samples were subjected to gel separation, membrane transferring, immunoblotting with the primary and secondary antibodies and scanning by Odyssey infrared fluorescent scanner (LI-COR Biosciences). .. The pixel density of every band was quantified using ImageJ software (version 1.48v, NIH, Bethesda, MD, USA) for statistical analysis.

    Membrane:

    Article Title: Chemical proteomics reveals sinomenine’s anti-inflammatory mechanism through serum protein covalent modification
    Article Snippet: .. After mixture and denaturation by boiling for 5 min, the plasma samples were subjected to gel separation, membrane transferring, immunoblotting with the primary and secondary antibodies and scanning by Odyssey infrared fluorescent scanner (LI-COR Biosciences). .. The pixel density of every band was quantified using ImageJ software (version 1.48v, NIH, Bethesda, MD, USA) for statistical analysis.

    Transferring:

    Article Title: Chemical proteomics reveals sinomenine’s anti-inflammatory mechanism through serum protein covalent modification
    Article Snippet: .. After mixture and denaturation by boiling for 5 min, the plasma samples were subjected to gel separation, membrane transferring, immunoblotting with the primary and secondary antibodies and scanning by Odyssey infrared fluorescent scanner (LI-COR Biosciences). .. The pixel density of every band was quantified using ImageJ software (version 1.48v, NIH, Bethesda, MD, USA) for statistical analysis.

    Western Blot:

    Article Title: Chemical proteomics reveals sinomenine’s anti-inflammatory mechanism through serum protein covalent modification
    Article Snippet: .. After mixture and denaturation by boiling for 5 min, the plasma samples were subjected to gel separation, membrane transferring, immunoblotting with the primary and secondary antibodies and scanning by Odyssey infrared fluorescent scanner (LI-COR Biosciences). .. The pixel density of every band was quantified using ImageJ software (version 1.48v, NIH, Bethesda, MD, USA) for statistical analysis.

    Incubation:

    Article Title: Cryptolepine suppresses breast adenocarcinoma via inhibition of HIF-1 mediated glycolysis.
    Article Snippet: .. After 1 h of incubation with the corresponding secondary antibody, the immunocomplexed bands were visualized by Odyssey infrared fluorescent scanner (LI-COR Biosciences). β-actin served as an internal control. ..

    Article Title: Non-classical cardenolides from Calotropis gigantea exhibit anticancer effect as HIF-1 inhibitors.
    Article Snippet: Six new non-classical cardenolides (1–6), and seventeen known ones (7–23) were isolated from Calotropis gigantea.. All cardenolides showed inhibitory effect on hypoxia inducible factor-1 (HIF-1) transcriptional activity with IC50 of 8.85 nM–16.69 μM except 5 and 7.. The novel 19-dihydrocalotoxin (1) exhibited a comparable HIF-1 inhibitory activity (IC50 of 139.57 nM) to digoxin (IC50 of 145.77 nM), a well-studied HIF-1 inhibitor, and 11, 12, 14, 16 and 19 presented 1.4–15.4 folds stronger HIF-1 inhibition than digoxin.

    Article Title: Coibamide A Targets Sec61 to Prevent Biogenesis of Secretory and Membrane Proteins
    Article Snippet: For Western blotting, proteins were transferred to nitrocellulose membranes (Bio-Rad). .. Following blocking of the membranes with Odyssey Blocking Buffer (PBS; LI-COR Biosciences) or 5% (w/v) nonfat dry milk in 50 mM Tris-HCl, pH 7.4, 150 mM NaCl (TBS) plus 0.1% Tween-20 (TBS-Tween), the membranes were first incubated with the appropriate primary antibodies and then with the appropriate secondary antibodies and finally imaged on an Odyssey infrared fluorescent scanner (LI-COR Biosciences) or a MyECL image analysis system (Thermo Fisher Scientific). ..

    Article Title: Systemic pharmacological verification of Guizhi Fuling decoction in treating endometriosis-associated pain.
    Article Snippet: Ethnopharmacological relevance: Guizhi Fuling decoction (GZFL decoction) is a famous formula in the Synopsis of the Golden Chamber, which has a long history in treating endometriosis.. However, its exact mechanism remains

    Control:

    Article Title: Cryptolepine suppresses breast adenocarcinoma via inhibition of HIF-1 mediated glycolysis.
    Article Snippet: .. After 1 h of incubation with the corresponding secondary antibody, the immunocomplexed bands were visualized by Odyssey infrared fluorescent scanner (LI-COR Biosciences). β-actin served as an internal control. ..

    Blocking Assay:

    Article Title: Coibamide A Targets Sec61 to Prevent Biogenesis of Secretory and Membrane Proteins
    Article Snippet: For Western blotting, proteins were transferred to nitrocellulose membranes (Bio-Rad). .. Following blocking of the membranes with Odyssey Blocking Buffer (PBS; LI-COR Biosciences) or 5% (w/v) nonfat dry milk in 50 mM Tris-HCl, pH 7.4, 150 mM NaCl (TBS) plus 0.1% Tween-20 (TBS-Tween), the membranes were first incubated with the appropriate primary antibodies and then with the appropriate secondary antibodies and finally imaged on an Odyssey infrared fluorescent scanner (LI-COR Biosciences) or a MyECL image analysis system (Thermo Fisher Scientific). ..



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    LI-COR odyssey infrared laser fluorescence scanner
    (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular <t>fluorescence</t> complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.
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    LI-COR infrared fluorescence scanner
    (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular <t>fluorescence</t> complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.
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    LI-COR li cor odyssey infrared fluorescence scanner
    (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular <t>fluorescence</t> complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.
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    Image Search Results


    (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular fluorescence complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.

    Journal: Cell reports

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry

    doi: 10.1016/j.celrep.2025.116903

    Figure Lengend Snippet: (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular fluorescence complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.

    Article Snippet: Co-detection was performed using IRDye 700 and 800-labeled secondary antibodies (926–68070 and 926–32211, Li-Cor) under the Odyssey infrared laser fluorescence scanner (Li-Cor) and quantified using ImageJ.

    Techniques: Bimolecular Fluorescence Complementation Assay, Fluorescence, Shear, Expressing, Control, Flow Cytometry